Development of enucleated mouse oocytes reconstituted with embryonic nuclei
نویسندگان
چکیده
منابع مشابه
Development of mouse embryonic nuclei transferred to enucleated oocytes and zygotes.
The present study was conducted to examine the development of nuclear transplant embryos produced by transplanting nuclei to either oocytes or zygotes in the mouse. Metaphase II oocytes and one-cell zygotes were enucleated and fused with transferred nuclei from late two-, four- and eight-cell stage embryos. Enucleation of metaphase oocytes was achieved using the interference microscope without ...
متن کاملDevelopmental potential of selectively enucleated mouse zygotes reconstituted with embryonic cell, embryonic stem cell and somatic cell nuclei*
In an overwhelming majority of experiments, both mammalian embryonic and somatic cloning have relied on introducing exogenous nuclei into enucleated metaphase II (MII) oocytes. Since attempts at cloning using interphase zygotes as recipient cells have failed, these cells were – until quite recently – commonly regarded as poor recipients for nuclear transfer. However, we have recently shown that...
متن کاملMetaphase II nuclei generated by germinal vesicle transfer in mouse oocytes support embryonic development to term.
BACKGROUND Cytoplasmic defects are thought to cause aneuploidies in oocytes and embryos and oocyte 'reconstruction' by germinal vesicle (GV) transfer may circumvent such defects. In mice 'reconstructed' oocytes undergo meiosis and fertilize normally, but early embryonic development is compromised if their ooplasm matured in vitro. This study employs sequential MII spindle and/or pronucleus (PN)...
متن کاملSomatic and embryonic cell nucleus transfer into intact and enucleated immature mouse oocytes.
BACKGROUND The aim of our study was to evaluate the possibility of embryonic or somatic cell haploidization after fusion with intact or enucleated immature oocytes which were subsequently cultured in vitro. Embryonic or somatic cell nuclei do not undergo premature chromosome condensation when fused to intact or enucleated immature oocytes whose maturation is prevented by dibutyryl cyclic AMP (d...
متن کاملEnucleated Oocytes and Zygotes
The present study was conducted to examine the development of nucLear transplant embryos produced by transplanting nucLei to either oocytes or zygotes in the mouse. Metaphase II oocytes and one-cell zygotes were enucleated and fused with transferred nuclei from late two-, fourand eight-cell stage embryos. Enucleation of metaphase oocytes was achieved using the interference microscope without st...
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ژورنال
عنوان ژورنال: Reproduction
سال: 1991
ISSN: 1470-1626,1741-7899
DOI: 10.1530/jrf.0.0930165